The Biotechnology

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SISNATE BIOTECHNOLOGY®

The Sisnate® Biological Treatment consists of a support inoculated and activated with microbial consortia that were isolated in the laboratory without undergoing any type of genetic change in their DNA. It comprises seven different types of species of microorganisms in proportional units of volume, according to the customer's flow data. Reports issued by a laboratory accredited by INMETRO proved that these species do not pose a risk to the environment and humans after acute ecotoxicity tests in accordance with the ABNT NBR 12713/09 standard.

Contaminated water is collected in containers and in the last container and stage of natural biological processes the water leaves decontaminated and unpolluted for residential or industrial reuse, not requiring continuous and permanent maintenance as the installations require minimal technical support and results with up to 98% efficiency. in your process.

How it works?

It is necessary to understand about Microbial species

These species are responsible for the bioremediation of organic matter in solution, being one of the most efficient biological methods for decontaminating effluents. They act in different situations in the synthesis of water decontamination, such as:
• Fat consumption; • They feed and are voracious on organic mud; • They use nitrate to release nitrogen; • Solubilize phosphate; • They consume macro nutrients and malate as a carbon source; • Transform lipids into carbon dioxide, water and energy; • Break double and triple bonds between atoms; • Degrade BTEX, amines, fatty acids, glycerol and form endospores under critical food conditions.

BIOMASS INOCULATION AND BIOLOGICAL REACTIONS

Inoculation is a term that generally refers to the process of introducing a microorganism (such as bacteria, viruses, fungi) or a substance into an environment or organism for specific purposes, such as scientific research, industrial production, or microbiological experiments.

BIOLOGICAL REACTIONS - They occur through catalytic enzymes. Some factors can influence catalytic activity. For example: [ ] enzymatic, [ ] substrate, hydrogen potential and temperature.

BIOMASS INOCULATION - In bio boxes, degrading organic matter, nutrients, organic sludge and removing other dissolved compounds and contaminating agents present in the tributary.

NITRIFICATION AND DENITRIFICATION PROCESS

NITRIFICATION - Nitrification is a chemical-biological process of formation of nitrite in the soil by the joint action of nitrifying chemosynthetic bacteria, by the action of converting ammonia into nitrate, Nitrosation → most of the ammonia is not absorbed by plants, being oxidized into nitrite by bacteria nitrous gases, using the energy released in this oxidation to produce organic compounds.

DENITRIFICATION - denitrification is the phenomenon of transformation of nitrates and other substances into nitrogen gas (N2) through the action of denitrifying bacteria. In the soil, in addition to nitrifying bacteria, there are other types, such as Pseudomonas denitrificans. In the absence of atmospheric oxygen, these bacteria use nitrate to oxidize organic compounds (anaerobic respiration).

KREBS CYCLE (Citric acid)

The Krebs cycle is one of the phases of cellular respiration that occurs in the mitochondrial matrix of eukaryotic organisms. The Krebs cycle, also called the citric acid cycle, or tricarboxylic acid cycle, is one of the phases of cellular respiration discovered by biochemist Hans Adolf Krebs in 1938. The Krebs cycle is defined as a metabolic route of catabolic and anabolic reactions of microorganisms with the purpose of oxidizing Acetyl-CoA to degrade fatty acids and oils, carbohydrates, amino acids to produce energy for your cells and assist in the respiratory process. After forming acetyl-CoA, a hydrolysis reaction will occur, in which coenzyme A will be released. At the same time, acetyl will react with oxaloacetic acid, which has 4 carbons, forming a 6-carbon molecule called citric acid (which is why the Krebs cycle is also called the citric acid cycle).

ADSORPTION AND BIOSORPTION

Sisnate® Technology is also covered by the phenomena of adsorption and Biosorption in the treatability of its effluent.

ADSORPTION Adhesion of fluid molecules or ions to a solid surface (support medium).
BIOSORPTION Mechanism of accumulation of metals by microorganisms, in extracellular or intracellular form by attracting metal cations into cells where anionic groups are found.

BIOLOGICAL FILTER

The final step for polishing the effluent. The filtering medium aims to form biofilm in the interstices and remove pathogens through the adsorption of microbial cells to the biofilms present in the filter, causing a natural decay of these populations as well as the whitening of the effluent. The efficiency of pathogen removal by Sisnate® Technology ranges from 95 to 99.99% without a disinfection process.

THE RESULT

Sisnate® stands out through the effectiveness of its work in favor of nature and offers a low-cost, high-yield service. Our goal is to have a practical and functional water reuse system.